| Concentric Sample Injector Reveals Room Temperature Structure of Ribosome-Antibiotic Complex | |
| Presenter | Raymond Sierra |
| Presentation Type | Poster |
| Full Author List |
Raymond Sierra, Cornelius Gati, Hartawan Laksmono, E. Han Dao, Sheraz Gul, Franklin Fuller, Jan Kern, Ruchira Chatterjee, Mohamed Ibrah |
| Affiliations |
Stanford University, SLAC National Accelerator Laboratory |
| Abstract | Serial Femtosecond X-ray crystallography has revolutionized structural biology by allowing data collection without structural radiation damage at ambient temperature. In this work a concentric-flow electrospin injector was used to deliver microcrystals of photosystem II and the small 30S ribosomal subunit. This injector allowed us to collect diffraction data from photosystem II complex beyond 3 Å resolution. We also obtained the first ambient-temperature X-ray crystal structure of 30S subunit bound to the antibiotic paromomycin at 3.4 Å resolution. Compared to previous cryo-cooled structures, this new structure showed that paromomycin binds to the decoding center in a different conformation. Our results demonstrate the feasibility of collecting near-atomic-resolution diffraction data from microcrystals of both photosystem II and ribosome-antibiotic complexes in their unperturbed native mother liquor at ambient temperature and at low flowrates. |