| Taking Snapshots of Photosynthetic Water Oxidation - Simultaneous Femtosecond X-ray Spectroscopy and Diffraction | |
| Abstract ID | 38 |
| Presenter | Jan Kern |
| Presentation Type | Poster |
| Full Author List | J. Kern, R. Alonso-Mori, R. Tran, J. Hattne, RJ Gildea, N. Echols, C. Glöckner, J. Hellmich, et al... |
| Affiliations | Lawrence Berkeley National Laboratory |
| Category | |
| Abstract |
X-ray free electron laser at Stanford, using sub-50fs X-ray pulses [4,5]. Simultaneously, Mn KβX-ray emission spectra of the same crystals were collected to monitor the intactness of the catalytic site [5]. We are using a visible-laser pump followed by the X-ray probe pulse to collect room temperature XRD and XES data from the higher S-states. From model systems we could show that using the sub-50 fs X-ray pulses of LCLS does not lead to a change of the measured X-ray emission spectrum, compared to cryogenic data measured at a synchrotron [6]. In addition, we show that the Mn4CaO5 cluster of PSII is not damaged within the time span (<50 fs) of the measurement [5]. Our studies open the possibility to follow changes at room temperature of the geometric and electronic structure of the catalytic site of PSII during the reaction cycle and further studies at different time points in the catalytic cycle are currently under way. https://conf-slac.stanford.edu/ssrl-lcls-2013/sites/conf-slac.stanford.e... |
| Footnotes |
[1] Umena et al, Nature 473 (2011) 55-60 |
| Funding Acknowledgement | |